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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
Data corrente: |
20/04/2016 |
Data da última atualização: |
21/07/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LACERDA, A. F.; SARTO, R. P. del; SILVA, M. S.; VASCONCELOS, E. A. R. de; COELHO, R. R.; SANTOS, V. O. dos; GODOY, C. V.; SEIXAS, C. D. S.; SILVA, M. C. M. da; GROSSI-DE-SA, M. F. |
Afiliação: |
ARIANE FERREIRA LACERDA, Universidade Federal do Rio Grande do Norte; RAFAEL PERSEGHINI DEL SARTO, Centro Universitário do Distrito Federal; MARILIA SANTOS SILVA, Cenargen; ERICO AUGUSTO ROSAS DE VASCONCELOS; ROBERTA RAMOS COELHO; VANESSA OLINTO DOS SANTOS, Universidade Federal do Rio Grande do Norte; CLAUDIA VIEIRA GODOY, CNPSO; CLAUDINE DINALI SANTOS SEIXAS, CNPSO; MARIA CRISTINA MATTAR DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
The recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium, Colletotrichum and Phakopsora. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
3 Biotech, v. 6, n. 1, p. 59, Feb. 2016. |
ISSN: |
2190-5738 |
DOI: |
10.1007/s13205-015-0320-7 |
Idioma: |
Inglês |
Conteúdo: |
Plant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian rust, as demonstrated by in planta assay. To our knowledge, this is the first report of a plant defensin active against an obligatory biotrophic phytopathogenic fungus. Results revealed the potential of rDrr230a as a candidate to be used in plant genetic engineering to control relevant cotton and soybean fungal diseases. MenosPlant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian ru... Mostrar Tudo |
Thesagro: |
Algodão; Doença de planta; Fungo; Soja. |
Thesaurus Nal: |
Cotton; Fungi; Plant diseases and disorders; Soybeans. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142476/1/the-recombinant-pea....pdf
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Marc: |
LEADER 02860naa a2200349 a 4500 001 2043768 005 2017-07-21 008 2016 bl uuuu u00u1 u #d 022 $a2190-5738 024 7 $a10.1007/s13205-015-0320-7$2DOI 100 1 $aLACERDA, A. F. 245 $aThe recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium, Colletotrichum and Phakopsora.$h[electronic resource] 260 $c2016 520 $aPlant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian rust, as demonstrated by in planta assay. To our knowledge, this is the first report of a plant defensin active against an obligatory biotrophic phytopathogenic fungus. Results revealed the potential of rDrr230a as a candidate to be used in plant genetic engineering to control relevant cotton and soybean fungal diseases. 650 $aCotton 650 $aFungi 650 $aPlant diseases and disorders 650 $aSoybeans 650 $aAlgodão 650 $aDoença de planta 650 $aFungo 650 $aSoja 700 1 $aSARTO, R. P. del 700 1 $aSILVA, M. S. 700 1 $aVASCONCELOS, E. A. R. de 700 1 $aCOELHO, R. R. 700 1 $aSANTOS, V. O. dos 700 1 $aGODOY, C. V. 700 1 $aSEIXAS, C. D. S. 700 1 $aSILVA, M. C. M. da 700 1 $aGROSSI-DE-SA, M. F. 773 $t3 Biotech$gv. 6, n. 1, p. 59, Feb. 2016.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
10/09/2015 |
Data da última atualização: |
27/01/2016 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
BARBOSA, B. D. R.; TEIXEIRA, R. P.; CHAVES, A. R. de M.; BASSOI, L. H. |
Afiliação: |
BRUNO DJVAN RAMOS BARBOSA, Bolsista Pibic/Facepe; RAFAEL POMBO TEIXEIRA, Bolsista Capes; AGNALDO RODRIGUES DE MELO CHAVES, CPATSA; LUIS HENRIQUE BASSOI, CPATSA. |
Título: |
Avaliação da dinâmica da água no solo, evapotranspiração, estado hídrico e produção da videira de vinho irrigada: sétimo ciclo de produção. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 10., 2015, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2015. |
Páginas: |
p. 88-92 |
Descrição Física: |
1 CD-ROM. |
Série: |
(Embrapa Semiárido. Documentos, 264). |
Idioma: |
Português |
Conteúdo: |
Este trabalho foi desenvolvido com objetivo de avaliar diferentes estratégias de irrigação na produção de uvas de vinhos da cultivar Syrah/Paulsen 1103 em Petrolina, PE, no Submédio do Vale do São Francisco. |
Palavras-Chave: |
Deficit hídrico; Ecofisiologia. |
Thesagro: |
Evapotranspiração; Irrigação; Uva; Viticultura; Vitis Vinifera. |
Thesaurus NAL: |
Grapes; Irrigation. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129387/1/Bruno-Djavan.pdf
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Marc: |
LEADER 01145nam a2200277 a 4500 001 2023687 005 2016-01-27 008 2015 bl uuuu u00u1 u #d 100 1 $aBARBOSA, B. D. R. 245 $aAvaliação da dinâmica da água no solo, evapotranspiração, estado hídrico e produção da videira de vinho irrigada$bsétimo ciclo de produção. 260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 10., 2015, Petrolina. Anais... Petrolina: Embrapa Semiárido$c2015 300 $ap. 88-92$c1 CD-ROM. 490 $a(Embrapa Semiárido. Documentos, 264). 520 $aEste trabalho foi desenvolvido com objetivo de avaliar diferentes estratégias de irrigação na produção de uvas de vinhos da cultivar Syrah/Paulsen 1103 em Petrolina, PE, no Submédio do Vale do São Francisco. 650 $aGrapes 650 $aIrrigation 650 $aEvapotranspiração 650 $aIrrigação 650 $aUva 650 $aViticultura 650 $aVitis Vinifera 653 $aDeficit hídrico 653 $aEcofisiologia 700 1 $aTEIXEIRA, R. P. 700 1 $aCHAVES, A. R. de M. 700 1 $aBASSOI, L. H.
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